LOCUS       sequence-107826-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector compatible with Flp-mediated (Flp-in) transgene 
            integration that allows for conditional cDNA and Luc expression upon
            integration. The vector contains an FRT site and two mut loxP 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pFrt-invCAG-ires-Luc
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(253..318,319..356)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    469..590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(506..525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     560..579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagacctgca ggtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
      301 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtatgc
      361 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
      421 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
      481 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
      541 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
      601 atgctagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      661 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tg
//
