LOCUS       sequence-10783-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PIK3CB
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             303..311
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cacggtttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagt tggcatgtgc ttcagtttcc taatgcctcc
      121 tgctatggca gacatccttg acatctgggc ggtggattca cagatagcat ctgatggctc
      181 catacctgtg gatttccttt tgcccactgg gatttatatc cagttggagg tacctcggga
      241 agctaccatt tcttatatta agcagatgtt atggaagcaa gttcacaatt acccaatgtt
      301 caacctcctt atggatattg actcctatat gtttgcatgt gtgaatcaga ctgctgtata
      361 tgaggagctt gaagatgaaa cacgaagact ctgtgatgtc agaccttttc ttccagttct
      421 caaattagtg acaagaagtt gtgacccagg ggaaaaatta gactcaaaaa ttggagtcct
      481 tataggaaaa ggtctgcatg aatttgattc cttgaaggat cctgaagtaa atgaatttcg
      541 aagaaaaatg cgcaaattca gcgaggaaaa aatcctgtca cttgtgggat tgtcttggat
      601 ggactggcta aaacaaacat atccaccaga gcatgaacca tccatccctg aaaacttaga
      661 agataaactt tatgggggaa agctcatcgt agctgttcat tttgaaaact gccagacgtg
      721 tttagctttc aagtgtctcc taatatgaat cctatcaaag taaatgaatt ggcaatccaa
      781 aaacgtttga ctattcatgg gaaggagatg agttagcccc tatgattatg tgttgcagtc
      841 agcggagagt agaatatgtt tttggtgatc atccactatt cagttccagt atatccggaa
      901 ctgtgtgatg aacagaag
//
