LOCUS       sequence-107832-         340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the Col1a1 locus that contains a Neo resistance
            cassette flanked by FRT sites, followed by an ATG-less Hygromycine 
            resistance gene. The homology arms match the sequence of FVB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pCol1a1-frt (FVB)
FEATURES             Location/Qualifiers
     source          1..340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttggagctgg gcgcgccact agtgcggggg
       61 actttgggat gcttgtcttc ttccacacca gcccctcctg gttttcccaa gtgtctctct
      121 gctcagcttt aacccatcag cctgcacgga taaacttgta agacttgtat gtccagaggc
      181 tgtgatctcc cacctatgcc acaccaaacc ggaaggggcc tgtggacata gatgtctttg
      241 cttacaggta agtgggcaca gaaagagaca cattcactca ggtccccact gtgacaccca
      301 cacggggcca cccacagctc ccattcactc cctcacgtta
//
