LOCUS       sequence-107837-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the Col1a1 locus that contains a Neo resistance
            cassette flanked by FRT sites, followed by an ATG-less Hygromycine 
            resistance gene. The homology arms match the sequence of FVB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pCol1a1-frt (FVB)
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..39
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        21..39
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtacgggc
       61 cggccctcga ggtcgcactg gtgacagcgg ccctgctgta tgtagcccca catcctctgc
      121 ctcatgaccc tcccccaaga agacttgaat acctggaaac cttgatctcc ttcctctaca
      181 cagggtcccc ccggccctcc tggaccccct ggccctcctg gacctcccag tggcggttat
      241 gacttcagct tcctgcctca gccacctcaa gagaagtctc aagatggtgg ccgctactac
      301 cgggccgatg atgctaacgt ggttcgtgac cgtgaccttg aggtggacac caccctcaag
      361 agcctgagtc agcagattga gaacatccgc agccccgaag gcagccgcaa gaaccctgcc
      421 cgcacatgcc gcgacctcaa gatgtgccac tctgactgga agagcggtaa ggattaaccc
      481 cagccacctc cc
//
