LOCUS       sequence-107839-         507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the Col1a1 locus that contains a Neo resistance
            cassette flanked by FRT sites, followed by an ATG-less Hygromycine 
            resistance gene. The homology arms match the sequence of FVB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pCol1a1-frt (FVB)
FEATURES             Location/Qualifiers
     source          1..507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..150)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     282..301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
       61 ttctcgacag acgtcgcggt gagttcaggc tttttggcca aggaagttcc tatactttct
      121 agagaatagg aacttcggaa taggaacttc taggtacgtg aaccatcacc ctaatcaagt
      181 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      241 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      301 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      361 gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
      421 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
      481 tcctccccct tgctgtcctg ccccacc
//
