LOCUS       sequence-107842-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conservative version of OmpA Redesign 4 with G99I mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Stapleton JA, Whitehead TA, Nanda V
  TITLE     Computational redesign of the lipid-facing surface of the outer 
            membrane protein OmpA.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
  PUBMED    26199411
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Jul 21. pii: 201501836."
COMMENT     Sequence Label: pJexpress414_OmpA171_R4cons_G99I
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..63
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     CDS             577..594
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             595..612
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaaaaaga ccgcaatcgc catcgcagtg gcactggctg gtttcgctac cgtagcgcag
       61 gccgcgccga aagacaacac cttctatctc ggcctgaaaa tggggatcag ccagtatcac
      121 gatacgggat ttattaacaa taatggtccg acgcatgaaa atcagctggg gatcggcgca
      181 tttggaggta tccaggtcaa tccctacgtt ggcctggaac tcggtgtcga cgtgctgggt
      241 cgtatgccct acaaaggcag tgtggaaaat ggggcgtata aagcacaagg gtatcaagca
      301 actcttaaga tgggcgtgcc gattaccgat gacgtggacg tatatctgcg tttaggcatc
      361 atggtatggc gtgcagatac caaatctaat gtttatggca aaaaccatga tactggcgtt
      421 agcccagtga tggcaggtgg ctctgaatat gcaattaccc ctgaaatcgc aatgcgcctc
      481 gaaacacaat ggactaacaa tattggtgat gcgcacacca ttggtacccg tccggacaat
      541 ctgatgctgt ctctgggtct gtcctaccgt ttcggttgtt gccctggttg ctgtcaccat
      601 caccaccatc actaa
//
