LOCUS       sequence-107845-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for the Col1a1 locus that contains a Neo resistance
            cassette flanked by FRT sites, followed by an ATG-less Hygromycine 
            resistance gene. The homology arms match the sequence of FVB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pCol1a1-frt (FVB)
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    282..403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(319..338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     373..392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
       61 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
      121 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
      181 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
      241 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
      301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
      421 cgagacctga ggcctgggag gtgttgccca tggatcctgg gaggttcatg agccctcaaa
      481 gggattcaag cagcagaaac agggactgag acatggagga ccaggttgct ccttggtcac
      541 acatggaggg gtagctggcc tctccccacc tctagcccaa agaaaccata atttagtata
      601 gaaggggcct tctaatgctg gggtgtcaca aggaggatga gaatttcttg ggtcaccctt
      661 gatctcacca gagagaggtg cccagccctc aaggaactct ccccgg
//
