LOCUS       sequence-107848-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector compatible with Flp-mediated (Flp-in) transgene 
            integration that allows for conditional Luciferase expression upon 
            integration. The vector contains an FRT site and two mutant loxP 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pFrt-invCAG-Luc
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    200..233
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    235..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    480..614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctcatctcga agtactcggc gtaggtaatg tccacctcga tatgtgcgtc ggtaaaggcg
       61 atggtgccgg gcaccagggc gtagcgcttc atggctttgt gcagctgctc gccggcggtc
      121 ccgtcttcga gtgggtagaa tggcgctggg cccttcttaa tgtttttggc atcttccatg
      181 gtggcagatc tggccggcca taacttcgta taatgtatgc tatacgaacg gtaggatcca
      241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      421 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcgctag
      481 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      541 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      601 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      661 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      721 tctagcatcc agacatgata agatacattg atgagtttgg acaa
//
