LOCUS       sequence-107865-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti vector for expression of inducible split Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: pLSC-5
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             171..440
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             450..506
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     1000..1020
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc
       61 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
      121 tcgagggtgg cggtggctcg ggtggcggtg gctcgggtgg cggtggctcg gagatgtggc
      181 acgagggcct ggaggaggcc agcagactgt acttcggcga gagaaacgtg aagggcatgt
      241 tcgaggtgct ggagcccctg cacgccatga tggagagagg cccccagacc ctgaaggaga
      301 ccagcttcaa ccaggcctac ggcagagacc tgatggaggc ccaggagtgg tgcagaaagt
      361 acatgaagag cggcaacgtg aaggacctga cccaggcctg ggacctgtac taccacgtgt
      421 tcagaagaat cagcaagcag ggatccggcg caacaaactt ctctctgctg aaacaagccg
      481 gagatgtcga agagaatcct ggaccgaccg agtacaagcc cacggtgcgc ctcgccaccc
      541 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
      601 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
      661 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
      721 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
      781 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
      841 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg
      901 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
      961 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     1021 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     1081 tgacccgcaa gcccgg
//
