LOCUS       sequence-107869-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti vector for expression of inducible split Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: pLSC-5
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(476..532)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(542..811)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc
       61 ccagaccctt gccctggtgg tcgggcgaga ctccgacggt ggccaggaac cacgcgggct
      121 ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac
      181 cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct
      241 ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt
      301 cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt
      361 ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta
      421 cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcggtc
      481 caggattctc ttcgacatct ccggcttgtt tcagcagaga gaagtttgtt gcgccggatc
      541 cctgcttgct gattcttctg aacacgtggt agtacaggtc ccaggcctgg gtcaggtcct
      601 tcacgttgcc gctcttcatg tactttctgc accactcctg ggcctccatc aggtctctgc
      661 cgtaggcctg gttgaagctg gtctccttca gggtctgggg gcctctctcc atcatggcgt
      721 gcaggggctc cagcacctcg aacatgccct tcacgtttct ctcgccgaag tacagtctgc
      781 tggcctcctc caggccctcg tgccacatct ccgagccacc gccacccgag ccaccgccac
      841 ccgagccacc gccaccctcg attttcttga agtagtcctc tttcagctgc ttcacggtca
      901 ctttccggtt ggtcttgaac agcaggtcca cgatggcctt tttctgctcg ccgctcagga
      961 aggcgggctt tctcattccc tcggtcacgt atttcacttt ggtcagctcg ttatacacgg
     1021 tgaagtactc gtacagcagg ctgtgcttgg gcagcacctt ctcgttgggc aggttcttat
     1081 cgaagttggt catccgctcg atgaagctct gggcggaagc gcc
//
