LOCUS       sequence-107880-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Shen WF, Krishnan K, Lawrence HJ, Largman C
  TITLE     The HOX homeodomain proteins block CBP histone acetyltransferase 
            activity
  JOURNAL   Mol Cell Biol. 2001 Nov;21(21):7509-22
  PUBMED    11585930
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2001-11"; volume: "21(21)"; pages: "7509-2"
COMMENT     Sequence Label: CBP-Bromo-SP65
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(269..285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(269..285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(282..304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    293..309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(317..323,324..341,342..347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(500..517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccgaattcc atggactaca aggacgacga tgacaagtct agactcgagg gatccccaga
       61 gtctttgcct tttcgtcagc ctgtagatcc tcagctccta ggaatcccag attattttga
      121 tatagtgaag aatcctatgg acctttctac catcaaacga aagctggaca cagggcaata
      181 tcaagaaccc tggcagtatg tggatgatgt ctggcttatg ttcaacaatg cgtggctata
      241 taatcgtaaa aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      481 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      661 tgctggcgtt tttcc
//
