LOCUS       sequence-107914-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains an Arabidopsis thaliana lcyE cDNA and produces an active 
            lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or 
            pAC-BETAipi-At to produce alpha-carotene in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A portfolio of plasmids for identification and analysis of 
            carotenoid pathway enzymes: Adonis aestivalis as a case study.
  JOURNAL   Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
  PUBMED    17634749
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photosynth 
            Res."; date: "2007-05"; volume: "92(2)"; pages: "245-59. Epub 2007 
            Jul 17"
COMMENT     Sequence Label: pY2F
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     121..137
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     122..138
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
       61 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
      121 tcgaggtcga cggtatcgat aagcttgata tcgaattcgc ggccgctaca aaaggaaata
      181 attagattcc tctttctgct tgctatacct tgatagaaca atataacaat ggtgtaagtc
      241 ttctcgctgt attcgaaatt atttggagga ggaaaatgga gtgtgttggg gctaggaatt
      301 tcgcagcaat ggcggtttca acatttccgt catggagttg tcgaaggaaa tttccagtgg
      361 ttaagagata cagctatagg aatattcgtt tcggtttgtg tagtgtcaga gctagcggcg
      421 gcggaagttc cggtagtgag agttgtgtag cggtgagaga agatttcgct gacgaagaag
      481 attttgtgaa agctggtggt tctgagattc tatttgttca aatgcagcag aacaaagata
      541 tggatgaaca gtctaagctt gttgataagt tgcctcctat atcaattggt gatggtgctt
      601 tggatctagt ggttattggt tgtggtcctg ctggtttagc cttggctgca gaatcagcta
      661 agcttggatt aaaagttgga ctcattggtc cagatcttcc ttttactaac aattacggtg
      721 tttgggaaga tgaattcaat gatcttgggc tgcaaaaatg tattgagcat gtttggagag
      781 agactattgt gtatctggat gatgacaagc ctattaccat tggccgtgct tatggaagag
      841 ttagtcgacg tt
//
