LOCUS       sequence-107916-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Macrolide biosensor. High copy plasmid that expresses GFP 
            proportional to macrolide concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-mphR
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             604..612
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agcactagtt attacgcgtg cggctgcagc agctggaagt cgtcgtgttc cgggaacatc
       61 aggcacagga tcgccgcgat ctgcgccaga acgtggtccg ccagttcacc gtccgggtca
      121 accgcccact gcatggtcgc acccgcgata acagagtgca gcagcagttc cgccgccgcc
      181 ggcgcacccg gcggcagacg tttacggata ccttcaacaa ccgcacggtt acgctggatc
      241 gccagggtac gcagttccgg aacctgcagt tcgtaccaag agatcaggta gttaacagag
      301 aagtcgttac gggtgttcat agaacgaacc agaacctgca ggaattccca cagaccctgc
      361 ggacccgcac cgatcgggat cgcgttcagg tagtgacgaa cctgttcaac accacgttcc
      421 atcatacgaa ccagcagggt gtcacggttg gtgaaacgct ggatcagcgc cgcacgagac
      481 agaccaactt ctttcgcaac accagacagg gtgaattcga tcggaccgca acgtttcaga
      541 acaacggtcg ccgcttccag aacttcgtcg tcagatttca gtttcggacg cggcatttgt
      601 attacctcct tatttcaaag ttaaacaaaa ttatttgtag agggaaaccg ttgtggtctc
      661 cctgaatata gcctacgagc cttatgcatg cccgtaaagt tatccagcaa ccactcatag
      721 acctagggca gcagataggg acgacgtggt gttagctgtg gtaaacgcca tgggcattaa
      781 aaaatgccct cttgggttat caagagggtc attatatttg ctgaggagtg ccgttaatta
      841 aggcgcaacg caattaatgt aagttagctc actcattagg
//
