LOCUS       sequence-107918-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mouse Nanog Q144A Mutants. It is modified from 
            pPyCAG-Nanog-IP (Addgene Plasmid #13838)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  TITLE     Plasmids of NANOG HD Mutants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPyCAG-Nanog(Q144A)-IP
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..33
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(962..986)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaattatcac aagtttgtac aaaaaagcag gctctgacat gagtgtgggt cttcctggtc
       61 cccacagttt gcctagttct gaggaagcat cgaattctgg gaacgcctca tcaatgcctg
      121 cagtttttca tcccgagaac tattcttgct tacaagggtc tgctactgag atgctctgca
      181 cagaggctgc ctctcctcgc ccttcctctg aagacctgcc tcttcaaggc agccctgatt
      241 cttctaccag tcccaaacaa aagctctcaa gtcctgaggc tgacaagggc cctgaggagg
      301 aggagaacaa ggtccttgcc aggaagcaga agatgcggac tgtgttctct caggcccagc
      361 tgtgtgcact caaggacagg tttcagaagc agaagtacct cagcctccag cagatgcaag
      421 aactctcctc cattctgaac ctgagctata agcaggttaa gacctggttt gcaaaccaaa
      481 ggatgaagtg caagcggtgg cagaaaaacc agtggttgaa gactagcaat ggtctgattc
      541 agaagggctc agcaccagtg gagtatccca gcatccattg cagctatccc cagggctatc
      601 tggtgaacgc atctggaagc ctttccatgt ggggcagcca gacttggacc aacccaactt
      661 ggagcagcca gacctggacc aacccaactt ggaacaacca gacctggacc aacccaactt
      721 ggagcagcca ggcctggacc gctcagtcct ggaacggcca gccttggaat gctgctccgc
      781 tccataactt cggggaggac tttctgcagc cttacgtaca gttgcagcaa aacttctctg
      841 ccagtgattt ggaggtgaat ttggaagcca ctagggaaag ccatgcgcat tttagcaccc
      901 cacaagcctt ggaattattc ctgaactact ctgtgactcc accaggtgaa atatgagact
      961 tacccagctt tcttgtacaa agtggtgata attccgccc
//
