LOCUS       sequence-107919-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Macrolide biosensor. Low copy plasmid that expresses GFP 
            proportional to macrolide concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-mphR
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             599..607
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tagttattac gcgtgcggct gcagcagctg gaagtcgtcg tgttccggga acatcaggca
       61 caggatcgcc gcgatctgcg ccagaacgtg gtccgccagt tcaccgtccg ggtcaaccgc
      121 ccactgcatg gtcgcacccg cgataacaga gtgcagcagc agttccgccg ccgccggcgc
      181 acccggcggc agacgtttac ggataccttc aacaaccgca cggttacgct ggatcgccag
      241 ggtacgcagt tccggaacct gcagttcgta ccaagagatc aggtagttaa cagagaagtc
      301 gttacgggtg ttcatagaac gaaccagaac ctgcaggaat tcccacagac cctgcggacc
      361 cgcaccgatc gggatcgcgt tcaggtagtg acgaacctgt tcaacaccac gttccatcat
      421 acgaaccagc agggtgtcac ggttggtgaa acgctggatc agcgccgcac gagacagacc
      481 aacttctttc gcaacaccag acagggtgaa ttcgatcgga ccgcaacgtt tcagaacaac
      541 ggtcgccgct tccagaactt cgtcgtcaga tttcagtttc ggacgcggca tttgtattac
      601 ctccttattt caaagttaaa caaaattatt tgtagaggga aaccgttgtg gtctccctga
      661 atatagccta cgagccttat gcatgcccgt aaagttatcc agcaaccact catagaccta
      721 gggcagcaga tagggacgac gtggtgttag ctgtggtaaa cgccatgggc attaaaaaat
      781 gccctcttgg gttatcaaga gggtcattat atttgctgag gagtgccgtt aattaaggcg
      841 caacgcaatt aatgt
//
