LOCUS       sequence-107922-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human GSK3 beta with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   He X, Saint-Jeannet JP, Woodgett JR, Varmus HE, Dawid IB
  TITLE     Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus 
            embryos.
  JOURNAL   Nature. 1995 Apr 13. 374(6523):617-22.
  PUBMED    7715701
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1995 Apr 13. 374(6523):617-22."
COMMENT     Sequence Label: HA GSK3 beta wt pcDNA3
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..90)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(70..96)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(531..542)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gggccctcta gatgcatgct cgactctaga ggatccgtcg ggactgtact agttgggacc
       61 acccaagctt gcgtaatctg gaacatcgta tgggtaacta gtggagttgg aagctgatgc
      121 agaagcagca ttattggtct gtccacggtc tccagtatta gcatctgacg ctgctgtggc
      181 atttgtgggg gttgaagcag ctgcttgaat ccgagcatga ggaggaataa ggatggtagc
      241 cagaggtgga ttacttgaca gttcttgagt ggtgaagttg aagagtgcag gtgtgtctcg
      301 cccatttggt agtttgacat ttgggtcccg taattcatca aaaaatgaat gtgcacaagc
      361 ttccagtggt gttagtcggg cagttggtgt atactccagc agacggctac acagtgcaat
      421 tgcctccggt ggagttcggg gtcggaagac cttagtccaa ggatgtgcct taatttgagg
      481 gaatttaaat tctgtgtagt ttgggttcat ttctctgatt tgctcccttg ttggagttcc
      541 caggaccttg attatttcta ccaactgatc cacaccacta tcccctggaa atattggttg
      601 tcctagtaac agctcagcca acacacagcc agcagaccat acatctatac tagaggtata
      661 atcagtggct ccaaagatca actctggtgc cctatagtac cgagaacaga tatacgaaac
      721 attgggttct cctcggacca gctgctttgc acttccaaag tcacagagtt ttaatacagc
      781 agtatcagga tccaacaaga ggttctgcgg tt
//
