LOCUS       sequence-107941-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCAS9-VP64 activator with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: dCAS9-VP64_GFP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     222..243
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(286..339)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(361..510)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(535..555)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
       61 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
      121 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
      181 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      241 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcactgggc caggattctc
      301 ctcgacgtca ccgcatgtta gcagacttcc tctgccctct ccactgccgc tagcgttaat
      361 cagcatgtcc aggtcgaaat catcaagggc gtcactgccg agcatgtcga ggtcaaagtc
      421 atcaagggca tccgaaccaa gcatgtcaag gtcaaaatca tcgagggcgt cacttcccag
      481 catatccaga tcaaaatcgt ccaatgcgtc agcccgtccg gatccagcgg ccgccacctt
      541 cctctttttc ttaggtccgc tacctcctcc tccgcttcct cctcctccgc ttccacctcc
      601 tccagcgctg tcgcctccca gctgagacag gtcgatccgt gtctcgtaca ggccggtgat
      661 gctctggtgg atcagggtgg cgtccagcac ctctttggtg ctggtgtacc tcttccggtc
      721 gatggtggtg tcaaagtact tgaaggcggc aggggctccc agattggtca gggtaaacag
      781 gtggatgata ttctcggcct gctctctgat gggcttatcc cggtgcttgt tgtaggcgga
      841 cagcactttg tccagattag cgtcggccag gtcactctct tggagaactc gctgatctgc
      901 tcgatgatct cgtccaggta gtgcttgtgc tgt
//
