LOCUS       sequence-107942-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCAS9-VP64 activator with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: dCAS9-VP64_GFP
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..56
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catttcaggt gtcgtgacgt acggccacca tgagccccaa gaagaagaga aaggtggagg
       61 ccagcgacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
      121 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      181 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      241 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      301 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
      361 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
      421 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
      481 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
      541 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
      601 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
      661 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
      721 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca
      781 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
      841 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
      901 cccagatcgg cgaccagtac gccgacctgt ttctggccgc
//
