LOCUS       sequence-107943-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 
            3rd generation lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: MS2-P65-HSF1_GFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(99..470)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg ccgctagcgg agacagtggg gtccttggct
      121 ttgggaggct ccgagcctgt cagcagggag atggtggggt cctcggcgaa gccgtcccct
      181 tcggagaagt aggagccctc tcccagctca aacagcaccg gcaggtcgtt gctcccggtg
      241 tccacggagc cggggtccag caggaacagc ggctgcgctg tgtagtgcac cagctgcttc
      301 cctgaatccg ggctgctgtt ctctgcctcg ggaggcctgg ggggctcctg gggagacagg
      361 agctcttgga tactggccag gctgctgtca aggtcaggca ggctcatgtc gggcacggtc
      421 accgaggggc tgaacaggtc cagcagggca ctggtgtcca cgctgaagcc gcttccacct
      481 cctccctgcc cactagagga aatctgtgac agcagggcac taaagtccat atcagcgatg
      541 cttgagaagt cttcatctcc ggacagccca ttaggcaggc cgctggttcc caggggagtt
      601 ggagcggggt cggggggccg ctggctgccg gtcaccagcc gggtaatggc ttcggggtac
      661 tccatcagca ttggttcggc tgtactatga gacatggaca cgccctgatt cagcagctgc
      721 tgaaactcag agttgtccac ggaggccaga tctgtgaaca ctccgggatc ggtgctgttc
      781 cccagcagag ctcccaggtc ctcatcagcg tcgaactgca ggtgcagcag agcttcactc
      841 agagtcccct cgccggcctg tgtagacttg ggcactggag cgctca
//
