LOCUS       sequence-107944-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 
            3rd generation lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: MS2-P65-HSF1_GFP
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..419
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             474..494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catttcaggt gtcgtgacgt acggccacca tggcttcaaa ctttactcag ttcgtgctcg
       61 tggacaatgg tgggacaggg gatgtgacag tggctccttc taatttcgct aatggggtgg
      121 cagagtggat cagctccaac tcacggagcc aggcctacaa ggtgacatgc agcgtcaggc
      181 agtctagtgc ccagaagaga aagtatacca tcaaggtgga ggtccccaaa gtggctaccc
      241 agacagtggg cggagtcgaa ctgcctgtcg ccgcttggag gtcctacctg aacatggagc
      301 tcactatccc aattttcgct accaattctg actgtgaact catcgtgaag gcaatgcagg
      361 ggctcctcaa agacggtaat cctatccctt ccgccatcgc cgctaactca ggtatctaca
      421 gcgctggagg aggtggaagc ggaggaggag gaagcggagg aggaggtagc ggacctaaga
      481 aaaagaggaa ggtggcggcc gctggatccc cttcagggca gatcagcaac caggccctgg
      541 ctctggcccc tagctccgct ccagtgctgg cccagactat ggtgccctct agtgctatgg
      601 tgcctctggc ccagccacct gctccagccc ctgtgctgac cccaggacca ccccagtcac
      661 tgagcgctcc agtgcccaag tctacacagg ccggcgaggg gactctgagt gaagctctgc
      721 tgcacctgca gttcgacgct gatgaggacc tgggagctct gctggggaac agcaccgatc
      781 ccggagtgtt cacagatctg gcctccgtgg acaactctga gtttcagcag ctgctgaatc
      841 agggcgtgtc catgtctcat agtacagccg aaccaatgct gatggagtac cccgaagcca
      901 ttacccggct ggtgaccggc agccagcggc cccccgaccc cgctccaact cccctgggaa
//
