LOCUS       sequence-107947-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCG150
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(150..455)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(252..271)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
       61 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg tcgacctgca
      121 gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg
      181 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
      241 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
      301 gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag ggggatcacc
      361 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
      421 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcggc
      481 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
      541 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccgaacc
      601 ggagatattg acatcatata tgcctt
//
