LOCUS       sequence-107954-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Cre recombinase reporter; 
            EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked 
            RFP-STOP cassette and switches fluorescence from red (RFP) to green 
            (GFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: Cre Reporter
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..151
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(238..271)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      322..331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(328..330,331..333,334..846)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(373..394)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(634..653)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgctgaagg gcgagatcca caaggcgctg aagctgaagg gcggcggcca ctacctggtg
       61 gagttcaagt caatctacat ggccaagaag cccgtgaagc tgcccggcta ctactacgtg
      121 gactccaagc tggacatcac ctcccacaac gaggactaca ccgtggtgga gcagtacgag
      181 cgcgccgagg cccgccacca cctgttccag tagagctagc tgaataaggc cgctcgaata
      241 acttcgtata gcatacatta tacgaagtta ttcgagtcta gagggcccgt tagatctctc
      301 gaggttaacg aattcgacta cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      361 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      421 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      481 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      541 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      601 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      661 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      721 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      781 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      841 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      901 ggccccgtgc tgctgcccga caaccactac ctgagcaccc
//
