LOCUS       sequence-107955-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Cre recombinase reporter; 
            EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked 
            RFP-STOP cassette and switches fluorescence from red (RFP) to green 
            (GFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: Cre Reporter
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(73..585,586..588,589..591))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     266..285
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     525..546
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      588..597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    648..681
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(768..791)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
       61 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
      121 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      181 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      241 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      301 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      361 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      421 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      481 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      541 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgta
      601 gtcgaattcg ttaacctcga gagatctaac gggccctcta gactcgaata acttcgtata
      661 atgtatgcta tacgaagtta ttcgagcggc cttattcagc tagctctact ggaacaggtg
      721 gtggcgggcc tcggcgcgct cgtactgctc caccacggtg tagtcctcgt tgtgggaggt
      781 gatgtccagc ttggagtcca cgtagtagta gccgggcagc ttcacgggct tcttggccat
      841 gtagattgac ttgaactcca ccaggtagtg gccgccgccc tt
//
