LOCUS       sequence-107975-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-HA
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             68..133
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             140..160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgccatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
      121 tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggccgcggaa tggataagaa
      181 atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
      241 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
      301 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
      361 caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
      421 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
      481 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
      541 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
      601 ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
      661 tcgtggtcat tttttgattg a
//
