LOCUS       sequence-107976-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-HA
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..40)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(59..79)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taccgattca agaagcgtag tccggaacgt cgtacgggta gttaattaag gcgcgcccaa
       61 ctttgcgttt ctttttcggg ctagccttgg atccggcaat tgggtcacct cctagctgac
      121 tcaaatcaat gcgtgtttca taaagaccag tgatggattg atggataaga gtggcatcta
      181 aaacttcttt tgtagacgta tatcgtttac gatcaattgt tgtatcaaaa tatttaaaag
      241 cagcgggagc tccaagattc gtcaacgtaa ataaatgaat aatattttct gcttgttcac
      301 gtattggttt gtctctatgt ttgttatatg cactaagaac tttatctaaa ttggcatctg
      361 ctaaaataac acgcttagaa aattcactga tttgctcaat aatctcatct aaataatgct
      421 tatgctgctc cacaaacaat tgtttttgtt cgttatcttc tggactaccc ttcaactttt
      481 cataatgact agctaaatat aaaaaattca catatttgct tggcagagcc agctcatttc
      541 ctttttgtaa ttctccggca ctagccagca tccgtttacg accgttttct aactcaaaaa
      601 gactatattt aggtagttta atgattaagt cttttttaac ttccttatat cctttagctt
      661 ctaaaaagtc aatcggattt ttttcaaagg aacttctttc cataattgtg atccctagta
      721 actctttaac ggattttaac ttcttcga
//
