LOCUS       sequence-107991-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MBK-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.16
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga tcgagggaag
       61 gatcacaagt ttgtacaaaa aagcaggctt aatgacgcaa tcgatcggtg gacccaataa
      121 acatttgtca gcgtcacact caacactaaa tactgcatca actcacgata tgatgcattc
      181 gaagattcca aagagcccga gcaatgagag tttaagccga tcacacacat cctcgtcggg
      241 aggcagtcaa ggtggacaca attcgaattc tggttcaaac tctggtttcc gaccggaaga
      301 tgctgtacag acgtttggcg cgaagctggt gccgtttgag aaaaatgaga tttacaacta
      361 tacacgggtc tttttcgtcg gatcacacgc taaaaagcag gccggtgtga ttggtggtgc
      421 gaataatggt ggatatgatg atgaaaatgg gtcctatcag cttgttgtac acgatcatat
      481 tgcgtaccgg tacgaggtgc tcaaggttat cggaaaagga tcgtttggac aggtaatcaa
      541 ggcattcgat cacaaatatc aacaatatgt ggctctgaag cttgtaagaa atgagaagcg
      601 attccatcgt caggctgacg aggagattcg aattcttgat catttgcggc ggcaggattc
      661 cgatggaaca cacaatatta tccatatgct cgactatttc aatttccgta atcacaagtg
      721 catcacgttt gagcttctca gcatcaacct ttacgagttg atcaagagga acaagttcca
      781 aggattctca ctgatgctcg tgagaaaatt cgcttattct atgctcttgt gtctggatct
      841 tttacaaaag aacaggctga ttcattgtga tttgaagccg gaaaatgttc tgttgaagca
      901 acaaggacgg agtggaataa aagtcatcga ttt
//
