LOCUS       sequence-108-exp        4485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_81
FEATURES             Location/Qualifiers
     source          1..4485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(162..190)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     938..958
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1925..1947)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2047..2066
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2144..2162)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2230..2334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2335..2403,2404..3195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2553..2572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3366..3954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3855..3874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4108..4125
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4242..4263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4278..4283,4284..4301,4302..4308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4316..4332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4321..4343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4340..4356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4340..4356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagagg atccccggga ttggccaaag gacccaaagg
       61 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tggagggtac
      121 cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      181 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      241 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      301 gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagccaacac
      361 ttgtcactac tttctgttat ggtgttcaat gcttctcgag atacccagat catatgaaac
      421 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      481 tcaaagatga cgggaactac aagacacgta agtttaaaca gttcggtact aactaaccat
      541 acatatttaa attttcaggt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      661 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      721 ttgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagaacttca
      781 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      841 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      901 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      961 ctgctgggat tacacatggc atggatgaac tatacaaata gcattcgtag aattccaact
     1021 gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc
     1081 atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat
     1141 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     1201 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     1261 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     1321 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     1381 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     1441 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     1501 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     1561 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     1621 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
     1681 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     1741 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     1801 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     1861 tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     1921 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1981 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2041 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     2101 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta
     2161 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3481 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3721 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     3781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4141 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4321 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt
     4381 taaacatgat cttactaact aactattctc atttaaattt tcagagctta aaaatggctg
     4441 aaatcactca caacgatgga tacgctaaca acttggaaat gaaat
//
