LOCUS       sequence-108008-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-PPTR-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.12
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga tcgagggaag
       61 gatcacaagt ttgtacaaaa aagcaggctt aatgcacgga agcgggcaca gtctaacggg
      121 cgctccgcat caaattccgc cgccacggac acagggagcc gccaccggtg gccaacaact
      181 ctcggcgaca gctaatcaat ttgttgataa aatcgatccg ttccataata aaagaggaac
      241 ttctagacga ttgcgcatca acaacagcag ccgatataat gttgattcgg ctcaagaact
      301 cgtacaattg gcgctcatca aagatactgc tgcaaatgag caaccggctc tcgttatcga
      361 aaagctcgtc caatgtcagc acgtattcga tttctatgat ccagtagctc aactgaaatg
      421 caaagaaatc aaaagagcag ctctaaatga gctcatcgat cacattacat cgacaaaagg
      481 agcaattgtt gagacaattt atccggctgt cattaaaatg gttgccaaga atatattccg
      541 tgtacttcca ccatcagaaa attgtgaatt tgatccagaa gaggatgaac cgactttaga
      601 agtctcgtgg ccacatttac agcttgtcta cgagctcttc ctgagattcc ttgagtcgcc
      661 agatttccaa gcttcaatcg gtaaaaagta tattgatcag agatttgtgc tcaagctgct
      721 cgatttattc gattcggagg atccacgtga acgagacttt ttgaagacgg ttcttcatcg
      781 tatttacgga aaatttttgg gccttcgggc ttttattcgc aaacatatca ataatatgtt
      841 tttgagattc gtatacgaaa cggactcatt caacggtgtc ggtgagcttc tcgagattct
      901 tggctcaatt ataaacggat t
//
