LOCUS       sequence-108009-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-PPTR-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.13
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..49
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaacaacaac aacaataaca ataacaacaa cctcgggatc gagggaagga tcacaagttt
       61 gtacaaaaaa gcaggcttaa tgttgagatc aaagaagaag gacaaggaaa atgggaaatc
      121 agagaaaaag gacaaagaga aggataagaa atcaatgaaa gatgacggtg ctggaagcag
      181 taaggcatct acaattccta cgatccgaac agaagatgtt ggaggagata tgattcctgc
      241 agatgcacca ccaccaacaa atattggccg aacaaacacg tacggaggag ggccagtgat
      301 accgcgaaga gagcgccgcc aatcaagtag catgttcaac atttcacaaa acagagagct
      361 gcaaagactt ccagctatca aagatgcgga tccgagcgag agagaaacac tgttcattca
      421 aaaacttcga caatgctgcg ttgtttttga ctttgccaat gatgctctca gcgatttaaa
      481 attcaaagaa gtcaaacggg cagctctaaa tgaactcgtc gaccacgttt ccggagctcc
      541 aaaaggatcg ctatctgatg ctgtctatcc agaagctatc ggcatgttct ccactaatct
      601 tttccgtcct ttgagcccac cgaccaatcc aattggtgct gaatttgacc ccgatgaaga
      661 cgaaccaaca ttagaagctg cctggccaca tctgcagctc gtctacgagt ttttcctgag
      721 attcctggag tgccccgatt ttcaatctca agttgcaaaa cgttacattg atcaaaattt
      781 cattcttcgt cttctcatga ttatggacag tgaagatcca cgtgaacgtg actttctgaa
      841 gacaacttta catcgaattt atggaaagtt cctcggacat cgtgcttaca ttcggaagca
      901 aatcaacaac attttctact cgttcatcta cgaa
//
