LOCUS       sequence-108011-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MEG-3 T541A S582A T605A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.28
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga tcgagggaag
       61 gatcacaagt ttgtacaaaa aagcaggctt aatgagttcc tcaaaacctt acccaagcgg
      121 tctgccaaac tcgagaagaa aaagaggagg tcgccgctca agtagtcgta gcaatcagga
      181 aagtgcatcc aacaacatgg aacaccaaat cactcttgac gaattgttca atccgatcgc
      241 aaaacaagac agtgctcagt caacaagccg tgaatatggt gcaaaatcgg ggatatctca
      301 tcacggatcc gtatcattca acgggaatac attcatgaat ggccaacagc tgaatcactc
      361 gatgactcga catggtcgag tattcaatca gtcgatgcat gcagctcaag gaaacggcag
      421 caacgccttt aacagtattc ctcctacggc ccccgtcttc tctgcagact tccgccgcaa
      481 cttgcaaaca cgcaatagct ccagctggta tgaacgaaga ttcccagtat ccacagatca
      541 ggacgacgtt cagcaatcaa acacaagacg tagtagaagt cgtcagaatg gacaacatgg
      601 cctaagcttc tcggatggtt cgaacaatta tggtcacgct gggaataagt cattcagtgt
      661 gagttccgtt cccgttgggt tccagaagca agaaaataat tctaagaaac tgcgacaaac
      721 caatgttcac cagcaatgtc ttggaaataa aagcttcaac gctcaagctg gagttcatgg
      781 acacgctttt aaaaaaggcc ataaggacaa taaaaatgca tctggaaagg aggttatcaa
      841 ttctagtctt gtccaaaagc acgatgctat taagtcgcgg aatttgaacc agagcttctc
      901 tggatttcct acacatgaaa cctcatcgat gaaaaatcaa c
//
