LOCUS       sequence-108012-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-PGL-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.02
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga tcgagggaag
       61 gatcacaagt ttgtacaaaa aagcaggctt aatggaggct aacaagcgag aaattgtgga
      121 tttcggtggg ctgagatcct actttttccc aaatttggcc cactacatca cgaagaatga
      181 cgaagaactg ttcaacaata catctcaggc caataagttg gctgctttcg tgttgggagc
      241 ttccaaagac gctcctggag acgaagacat tttggagatg atcttgccga acgacgctaa
      301 cgctgccgtt attgctgctg gcatggacgt ttgtcttctt ctcggagata aatttcgtcc
      361 gaaatttgat gctgctgctg agaaattgtc aggattggga catgctcatg atctggtttc
      421 tgtcatcgat gacgacaaga agctcggaat gctcgcccgt aaggccaagc tgaagaagac
      481 tgaagacgct aagattcttc aagctcttct caaagtaatc gcaatcgatg atgccgccga
      541 aaagtttgtt gagctcacgg aacttgtcag ccaactggat ctggactttg atgtttatgt
      601 cctaactaaa atcctcggac ttatctctga ggagactagc gacgaggtcg acattatccg
      661 tgataacgtc gtcaacgcat ttgatagctg caagccgttg ttgaaacagt tgatgttgga
      721 tggtccgaaa tctgaacctg ccgatccatt cattagtctc ctcatggatc cactcgaaga
      781 gtctgttggg aaagttgtga accatatcgc tcaacttttc gaggaagcga gtaaaaatga
      841 aggagacgag tcacttgtct tgagatctca
//
