LOCUS       sequence-108014-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The multiple cloning site of pGreenII0000 cloned into binary vector 
            pDHt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pDHt-KS
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    26..50
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     284..301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    418..439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(454..459,460..477,478..484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    492..508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     497..519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     516..532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     516..532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     551..571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        553..571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    584..691
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     608..624
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     608..624
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(658..674)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(659..675)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(699..718)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(700..718)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(728..745)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(728..744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(737..759)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata
       61 gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc
      121 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      181 agccttcatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      301 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      361 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      421 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      481 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      541 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg
      601 cggccgctct agaactagtg gatcccccgg gctgcaggaa ttcgatatca agcttatcga
      661 taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
      721 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      781 ttaatcgcct tgcagcacat
//
