LOCUS       sequence-108020-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OmpR constitutively from PompB97 and sfGFP under the 
            PompF146 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR59.4
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     232..249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(273..330)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(337..364)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(380..451)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcatcaaga ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa
       61 actgtccata tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg
      121 agtttttggt gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cctcgagatt
      181 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      241 gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga aaaagggcag
      301 ggtggtgaca ccttgccctt ttttgccgga tataaacgca gaaaggccca cccgaaggtg
      361 agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      421 agtctttcga ctgagccttt cgttttattt gatgcctggt ctagattatt atcatgcttt
      481 agagccgtcc ggtacaaaga cgtagcccag accccagacg gtctgaatgt aacgcggatg
      541 cgctggatct tcttccacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
      601 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
      661 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
      721 ctcgtcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
      781 taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc
      841 acggatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
      901 aatctccagg cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
      961 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
     1021 cat
//
