LOCUS       sequence-108022-         365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OmpR constitutively from PompB97 and sfGFP under the 
            PompF146 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR59.4
FEATURES             Location/Qualifiers
     source          1..365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(129..233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     298..316
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(320..363)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
       61 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg taaatgttga
      121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      241 cccatggtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      301 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      361 ttctg
//
