LOCUS       sequence-108024-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaR constitutively and sfGFP under the PcpcG2-172 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR58.6
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(263..290)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(306..377)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gttcttgcat gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      181 tgataccgct cgccgcagcc gaacgcctag gaagcttgca tgcctgcagg tcgactctag
      241 aggatccacg ggtacctata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc
      301 tagtagagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
      361 gcctttcgtt ttatttgatg cctggtctag attattatca tcatttgtac agttcatcca
      421 taccatgcgt gatgcccgct gcggttacga actccagcag aaccatatga tcgcgtttct
      481 cgttcggatc tttagacaga acgctttgcg tgctcagata gtgattgtct ggcagcagaa
      541 caggaccatc accgattgga gtgttttgct ggtagtgatc agccagctgc acgctgccat
      601 cctccacgtt gtggcgaatt ttaaaattcg ctttaatgcc atttttttgt ttatcggcgg
      661 tgatgtaaac attgtggctg ttaaaattgt attccagctt atggcccagg atattgccgt
      721 cttctttaaa gtcaatgcct ttcagctcaa tgcggtttac cagggtatcg ccttcaaatt
      781 tcacttccgc acgcgttttg tacgtgccgt catccttaaa ggaaatcgtg cgttcctgca
      841 catagccttc cggcatggcg gacttgaaga agtcatgctg cttcatatgg tccggataac
      901 gagcaaagca ctgaacacca taagtcagcg tcgttaccag agtcggccaa ggtaccggca
      961 gtttaccagt agtacagatg aacttcagcg tcagtttacc attagttgcg tcaccttcac
     1021 cctcgccacg cacgg
//
