LOCUS       sequence-108025-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaR constitutively and sfGFP under the PcpcG2-172 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 34)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR58.6
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..34
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      794..865
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      881..908
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tgacggctag ctcagtccta ggtacagtgc tagctgttaa acgctcctcg taagaacacg
       61 ggaaagcaat gagaattctt ttagtggagg atgatttgcc gctggcggaa acccttgctg
      121 aagcattgag tgaccagctt tacaccgttg atattgccac cgacgcttcc ctcgcctggg
      181 actatgcctc ccgactggaa tatgacctcg ttattttgga tgtgatgctg ccggagttgg
      241 acgggattac cctctgtcaa aaatggcgat cgcacagtta tttaatgcca attttgatga
      301 tgacagccag ggatacgatc aatgataaaa tcacgggctt ggatgcgggg gcggatgatt
      361 atgtggtcaa gccagtggat ttgggggagt tatttgccag ggtgcgagct ttgttgcgtc
      421 ggggttgtgc aacgtgccaa ccagttttag agtgggggcc aatcaggttg gatccaagca
      481 cctatgaagt tagttatgac aatgaggttt tgtctttgac ccgcaaggaa tacagcattc
      541 tggaattact actccgcaat ggccgtcggg tgctaagtcg gagcatgatt atcgatagta
      601 tctggaagtt ggagagtccc ccagaggaag atacggttaa ggtgcatgtg cggagtttgc
      661 gacaaaaatt aaaaagtgcc ggtttatcag cagatgccat tgaaacggtc catggcattg
      721 ggtatcgtct ggccaattta acggaaaaat ctttgtgcca agggaaaaac tagtaataat
      781 ctagaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tatagtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctccc
//
