LOCUS       sequence-108026-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaR constitutively and sfGFP under the PcpcG2-172 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR58.6
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             239..250
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             257..970
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctggttagc
       61 tagtcaagcc cattgtgctt ttctctatca acctcagctt acctgaaggg gtgaacaggt
      121 ctgggttaat tcatgttgcg aaatgtaaca gttttagtcg catcagctaa ctttccgatt
      181 tctttacgat tttctccccc ttttcttcaa ttttactttg ttaggatcgc atttttaaaa
      241 agaggagaaa tactagatgc gtaaaggcga agagctgttc actggtgtcg tccctattct
      301 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg
      361 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt
      421 accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc
      481 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga
      541 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga
      601 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa
      661 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga
      721 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag
      781 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct
      841 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg
      901 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga
      961 actgtacaaa tgatgataat aatctagacc aggcatcaaa taaaacgaaa ggct
//
