LOCUS       sequence-108030-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS, Ho1, and PcyA constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR43.6r
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             135..146
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(163..197)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      complement(314..341)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(357..428)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 accagcttac ggtaggaatt tttctcgaca acgcccttga ggaagcattt gacaaagccg
       61 acgttctccg ccatggagtg ggattttttc gtcccttccc gcaactggga agctaagttg
      121 acactcatgg tacctttctc ctctttaatg aattctgtgt gagctagcat tatacctagg
      181 actgagctag ctgtcagttt ttttgacggt aaagccaagc tgaaggctga caaaatctat
      241 actgttatat ccccctttcc tgggggactt aggcggttta atcgacacga attatgcagt
      301 gatttactat aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga
      361 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
      421 tttatttgat gcctggtcta gattattact aagctcgagg caaatggtta tagcgtttta
      481 aacagatagt aactgttgtt ccctggtcaa ctgcactttt tagcaagata ctgccactgt
      541 gtaagtcaac acatttcttg gcaaccatta aaccgagtcc tgttcccgta atatttctga
      601 catttttgcc ccgatgaaag ggttcaaaaa tttgcttttg gtcctctggc gaaatgccaa
      661 tgccctggtc ggtgacttca aaaataatct gttccgaatc taggcttagg gcaattttaa
      721 tctgccctcc cccgggagag tatttaatcg cattagataa cagattagat aaaatagacc
      781 gcactaacct ttcatccacc aatgccttcg tatcttgagc gctacaaata aagtcaaaat
//
