LOCUS       sequence-108032-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cph8, Ho1, and PcyA constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR33.4r
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..60)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             828..839
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
       61 atgcctggtc tagattattg gataacatca aataagactt ggctcatata ccgttccgcc
      121 caagcttccc caaaagcttt ttccagtacc cgacgggttt tatcattttt ctgttgttgt
      181 tggcagtaat gaatttgccc ctgacggtgc tccaaagttt gagcttcaga caagggttcg
      241 gcaacgatgg attggtgaca atgaatttgc aaaaagtcca ctaccctttg tacaaatctt
      301 tcttcttcag tgacattgct gggacggatg aataaacaat attcagaaaa tatttctccc
      361 caggggggca attcccgttg ttgctcaaat tctggctggc ctagctctgc caatgatttt
      421 tggtacgctg cgggcaattg gcgatcgctt tgggtggggg atagatccgc aatagccgca
      481 cttactccac cggggccggc cacaatgtca cagccaaaca aaggtagacc gtataaaggc
      541 tcaggaaaca ttacacagtg gagaatatcc aaccctttgc ccaccttggc caactccaaa
      601 tgcattttgc gaaactgggg cgtttggtag caccgatttt caatcactaa cttttcccct
      661 tccaggcggc cttctacgta gcccaaatcc tccggcaatt gatagggctt gaggggtaaa
      721 ctttgccaac tagcggcgat cgccagggcc aactgttgaa tcatcgggtt caacgtgggc
      781 atcagggaag aattggtcaa acttaaatca gtgacggcca tggatccttt ctcctcttta
      841 actagccttc ggaggtggcg aggccaactt cggtgctgcc ttgactgcgg cgacgggtga
      901 ggctgttgaa
//
