LOCUS       sequence-108033-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cph8, Ho1, and PcyA constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR33.4r
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        187..221
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     RBS             238..249
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggtggagtct ggcctcaaat acaaagtctg caaagtctcg aaatattaaa ccagagaata
       61 aataattaaa cgccaaaata tttctagcaa aaaccccagt tattaaaccg cctaagtccc
      121 ccaggaaagg gggatataac agtatagatt ttgtcagcct tcagcttggc tttaccgtca
      181 aaaaaactga cagctagctc agtcctaggt ataatgctag ctcacacaga attcattaaa
      241 gaggagaaag gtaccatgag tgtcaactta gcttcccagt tgcgggaagg gacgaaaaaa
      301 tcccactcca tggcggagaa cgtcggcttt gtcaaatgct tcctcaaggg cgttgtcgag
      361 aaaaattcct accgtaagct ggttggcaat ctctactttg tctacagtgc catggaagag
      421 gaaatggcaa aatttaagga ccatcccatc ctcagccaca tttacttccc cgaactcaac
      481 cgcaaacaaa gcctagagca agacctgcaa ttctattacg gctccaactg gcggcaagaa
      541 gtgaaaattt ctgccgctgg ccaagcctat gtggaccgag tccggcaagt ggccgctacg
      601 gcccctgaat tgttggtggc ccattcctac acccgttacc tgggggatct ttccggcggt
      661 caaattctca agaaaattgc ccaaaatgcc atgaatctcc acgatggtgg cacagctttc
      721 tatgaatttg ccgacattga tgacgaaaag gcttttaaaa atacctaccg tcaagctatg
      781 aatgatctgc ccattgacca agccaccgcc gaacggattg tggatgaagc caatgacgcc
      841 tttgccatga acatgaaaat gttcaacgaa cttgaaggca acctgatcaa ggcga
//
