LOCUS       sequence-108035-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target a DHFR-destabilized Cre recombinase gene to the stop codon of
            the mouse Rasgrf2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Rasgrf2-2A-dCre targeting vector
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(586..639)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 catgtccatc aggttcttgc gaacctcatc actcgttgca tcgaccggta atgcaggcaa
       61 attttggtgt acggtcagta aattggacat gctagctctc ctttccagga tctcaaagca
      121 ataactatga gaattctgag catcggcgtc gtgaaactcg gagaacacgc tttcccaatc
      181 gtcgggctca taatcaggaa aatgggtgtc cccttccacc tcagcgtcaa tgtgagtcag
      241 gtacagtttc tgggccttag gcaggaactg ctcgatcact ctccctccgc cgatgaccat
      301 gatctcgggc acatctccgc aagcggcaat ggcttcgtcc acacttttca cccatgtcac
      361 cctgtcgtcg gtagagggct gggagctcag aatgatgttc ttgcgtccag gcaggggccg
      421 tccaatggac tcccaagtgt gtctgcccat gatcactggc ttgttcaggg tgttcctctt
      481 gaaccaagcc agatcggcgg gcagattcca tggcatagcg ttttccatcc cgatcacgta
      541 gtccacggcc agagcggcaa tcagagagat cattgagcct ggggcagggc cgggattctc
      601 ttccacgtct ccgcatgtca gaagacttcc tctgccctcg ccgcttccag cagggagtcg
      661 aggttcaatt ttgagtgaca gctcatagag tgaatcttcg tctatgacaa gggctttgtc
      721 aagcaggtat tgtatgacct gaaa
//
