LOCUS       sequence-108041-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             167..190
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat accatgggca gcagccatca ccaccatcat catcaccttg aagtcctctt
      181 tcagggaccc atgacgggca acgccgggga gtggtgcctc atggaaagcg accccggggt
      241 cttcaccgag ctcattaaag gattcggttg ccgaggagcc caagtagaag aaatatggag
      301 tttagagcct gagaattttg aaaaattaaa gccagttcat gggttaattt ttcttttcaa
      361 gtggcagcca ggagaagaac cagcaggctc tgtggttcag gactcccgac ttgacacgat
      421 attttttgct aagcaggtaa ttaataatgc ttgtgctact caagccatag tgagtgtgtt
      481 actgaactgt acccaccagg atgtccattt aggcgagaca ttatcagagt ttaaagaatt
      541 ttcacaaagt tttgatgcag ctatgaaagg cttggcactg agcaattcag atgtgattcg
      601 acaagtacac aacagtttcg ccagacagca aatgtttgaa tttgatacga agacatcagc
      661 aaaagaagaa gatgcttttc actttgtcag ttatgttcct gttaatggga gactgtatga
      721 attagatgga ttaagagaag gaccgattga tttaggtgca tgcaatcaag atgattggat
      781 cagtgcagta aggcctgtca tagaa
//
