LOCUS       sequence-108045-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: 
            N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Integrin-Alpha2-N-18
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..619)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
       61 tcgcggccgc ttaacccagc ccaggcttgc ccagtctgtg gccctcgtgg gccagcagcc
      121 actctttcac ggcgagcccg ccctcgtagc cccccacgtc caggtcgccc tgcaccaccc
      181 ggtggcaggg gatcagaatg ggcacgggat ttccgctcag ggcggttttc acggcggcgg
      241 tggcggcggg attgccggcc agggcggcca ggtggctgta gctgatgacc tctccgaact
      301 tcaccacttt cagcagtttc cacagcacct ggcgggtaaa gctctcctgc tggaacactg
      361 ggtggtgcag ggctggcaca gggaactcct cgatggcctc aggctggtga aagtaggcgt
      421 tgagccaggc ggtggcctgc atcagtggct ctggtccgcc cagcacggcg gctggggcag
      481 gcacttccac ggcgtcggcg gcagatgttc ctttgcccag gaagatgata cggtgcaggc
      541 cctgttcgca cccagacagt tccagcttgc ccagagggct atccagggtg gtgcgcttca
      601 tttcgcagtc tttgtccatg gtggcgaccg gtggatcccg ggcccgcggt aaccccgctt
      661 ccgcctgcgc tgccgctact gagctctgtg gtctcatcaa tctcatctgg atttttggtc
      721 atcttttcat attttctttt gaagaagccg agcttccata aaattgcaac cagagctaac
      781 agcaaaaagg attccagcaa ttatactttc ctattataac tccctgtt
//
