LOCUS       sequence-108051-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat accatgggca gcagccatca ccatcatcac cacagccagg atccgaattc
      181 gagctcggcg cgcctgcagg tcgagatgac gggcaacgcc ggggagtggt gcctcatgga
      241 aagcgacccc ggggtcttca ccgagctcat taaaggattc ggttgccgag gagcccaagt
      301 agaagaaata tggagtttag agcctgagaa ttttgaaaaa ttaaagccag ttcatgggtt
      361 aatttttctt ttcaagtggc agccaggaga agaaccagca ggctctgtgg ttcaggactc
      421 ccgacttgac acgatatttt ttgctaagca ggtaattaat aatgcttgtg ctactcaagc
      481 catagtgagt gtgttactga actgtaccca ccaggatgtc catttaggcg agacattatc
      541 agagtttaaa gaattttcac aaagttttga tgcagctatg aaaggcttgg cactgagcaa
      601 ttcagatgtg attcgacaag tacacaacag tttcgccaga cagcaaatgt ttgaatttga
      661 tacgaagaca tcagcaaaag aagaagatgc ttttcacttt gtcagttatg ttcctgttaa
      721 tgggagactg tatgaattag atggattaag agaaggaccg attgatt
//
