LOCUS       sequence-10806-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-TPR
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatgatttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagtt ggcaccatgg cggcggtgtt gcagcaagtc
      121 ctggagcgca cggagctgaa caagctgccc aagtctgtcc agaacaaact tgaaaagttc
      181 cttgctgatc agcaatccga gatcgatggc ctgaaggggc ggcatgagaa atttaaggtg
      241 gagagcgaac aacagtattt tgaaatagaa aagaggttgt cccacagtca ggagagactt
      301 gtgaatgaaa cccgagagtg tcaaagcttg cggcttgagc tagagaaact caacaatcaa
      361 ctgaaggcac taactgagaa aaacaaagaa cttgaaattg ctcaggatcg caatattgcc
      421 attcagagcc aatttacaag aacaaaggaa gaattagaag ctgagaaaag agacttaatt
      481 agaaccaatg agagactatc tcaagaactt gaatacttaa cagaggatgt taaacgtctg
      541 aatgaaaaac ttaaagaaag caatacaaca aagggtgaac ttcagttaaa attggatgaa
      601 cttcaagctt ctgatgtttc tgttaagtat cgagaaaaac gcttggagca agaaaaggaa
      661 ttgctacata gtcagaatac atggctgaat acagagttga aaaccaaaac tgatgaactt
      721 ctggctcttg gaagagaaaa aggaatgaga ttctagagct taaatgtaat cttgaaaata
      781 aaaaagaaga ggtttctaga ctggaagaac aaatgaatgg cttta
//
