LOCUS       sequence-108073-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; mutation C1204R) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core (C1204R)
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..27)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      255..264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcgtagtcc ggaacgtcgt acgggtagtc ctggctctgc gtgtgcagct ccaccagcat
       61 gcacatggtg gaccactggg ctcttcggag tgaagagaac tccaggtgct tgtcccttgc
      121 cagcgtgaga aacgcatccc gaccatccat cagatcgcag gggatgagag gatcaggatc
      181 aacaatggga ggcagggagt tggcagcagg gccagcaatg aggcggatca caaagaagac
      241 ctctttatgc ttctccatgg tggcatatag tttctgtgag aggtcgttag atacattggg
      301 catcccgggt ttcttcttgt tgcccctact caggctgctc ttatttttgc tggttttctt
      361 attattcttc tttttagcat ttttgctgtc tcccttggtc acatctgtgc tttcattgct
      421 ggtgttttcc tctcgttttc tctcttcttc ctcctgttcc agttccttaa tgctttcttc
      481 cagaacattg ggccagaaat caccctcgaa ataaggcaat tcctttgcac ttgttaatct
      541 atcttcagta gcttgtttaa aaatatcctt gtagtcatgg acaatacgct ctgatacagc
      601 cttgtcaagc atttttttgt accattcctg cagtcgcttg ggcttgggta tcttctggtc
      661 aggaggatgg c
//
