LOCUS       sequence-108083-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core (Y1467F)
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             76..141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagcg ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat
      121 tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg ccgcggaatg
      181 gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc
      241 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
      301 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
      361 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
      421 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
      481 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat
      541 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
      601 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
      661 attaa
//
