LOCUS       sequence-108091-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 
            17-2, cloned into the BamHI site of pUC19..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Baudry B, Savarino SJ, Vial P, Kaper JB, Levine MM
  TITLE     A sensitive and specific DNA probe to identify enteroaggregative 
            Escherichia coli, a recently discovered diarrheal pathogen.
  JOURNAL   J Infect Dis. 1990 Jun;161(6):1249-51.
  PUBMED    2189007
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Infect 
            Dis."; date: "1990-06"; volume: "161(6)"; pages: "1249-51"
COMMENT     Sequence Label: pCVD432
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(796..812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(796..812)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(809..831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    820..836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tattatactc atttcttgca ataaatgctt atctgttttc gacacagact ctggcgaaag
       61 actgtatcat tgataatttc tttcagaaaa gcatccagtt taattcttat tctcttgata
      121 tcgaagagtt agatattaat aaacataaca atataaaaac gatgttacca gatataaata
      181 tagggttagg gcagtatata aacaacaatc aatggttctc atctattaca gacagccatt
      241 tttatttatc attatcctat aatcttctat cggcttatga agcaaaaatg cagaataata
      301 aattggatat tgctaattat ttaaaatata ttgaaatgct tagtgagagg aacaactaca
      361 taattaattt gttctcggaa attattaact ataagataaa aaaatctcac ctgatgttga
      421 tgctcgagag atataggaag ctcaataaag aatacgaaat tgcaaagcat aaaatgtcaa
      481 ttggattaat atctgctctt gatgtagaga tgatatataa tattttacaa aaaatcaggt
      541 ttgatattga tgtccttgag gaggaggaaa gtttactgtc agataaaatc tcgagagaat
      601 atcatgttcc tgagagtgca atcccagaca ttacatatca taagttaaaa gagtgtaaaa
      661 cagcggattt ctatacatta ttagctgaaa acaaaaaact caagattaag gctgctgata
      721 tagataatga tataaggaaa ctatctgaga tcctctagag tcgacctgca ggcatgcaag
      781 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      841 acacaacata cgagccgga
//
