LOCUS       sequence-108105-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutations 
            H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core
            (H1415A/E1423A/Y1424A/L1...
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..30)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      258..267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agaagcgtag tccggaacgt cgtacgggta gtcctggctc tgcgtgtgca gctccaccag
       61 catgcacatg gtggaccact gggctcttcg gagtgaagag aactccaggt gcttgtccct
      121 tgccagcgtg agaaacgcat cccgaccatc catcagatcg caggggatga gaggatcagg
      181 atcaacaatg ggaggcaggg agttggcagc agggccagca atgaggcgga tcacaaagaa
      241 gacctcttta tgcttctcca tggtggcata tagtttctgt gagaggtcgt tagatacatt
      301 gggcatcccg ggtttcttct tgttgcccct actcaggctg ctcttatttt tgctggtttt
      361 cttattattc ttctttttag catttttgct gtctcccttg gtcacatctg tgctttcatt
      421 gctggtgttt tcctctcgtt ttctctcttc ttcctcctgt tccagttcct taatgctttc
      481 ttccagaaca ttgggccaga aatcaccctc gaaataaggc aattcctttg cacttgttaa
      541 tctatcttca gtagcttgtt taaaaatatc cttgtagtca tggacaatac gctctgatac
      601 agccttgtca agcatttttt tgtaccattc ctgcagtcgc ttgggcttgg gtatcttctg
      661 gtcaggagga tggcaatgga agatataatc atc
//
