LOCUS       sequence-108106-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes N. meningiditis dCas9 with c-terminal fusion of human p300 
            HAT core (aa 1048-1664) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA3.3-Nm-dCas9-p300 Core
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg gactctagag gatcgaaccc ttgccaccat ggccgccttc
      121 aagcccaacc ccatcaacta catcctgggc ctggccatcg gcatcgccag cgtgggctgg
      181 gccatggtgg agatcgacga ggacgagaac cccatctgcc tgatcgacct gggtgtgcgc
      241 gtgttcgagc gcgctgaggt gcccaagact ggtgacagtc tggctatggc tcgccggctt
      301 gctcgctctg ttcggcgcct tactcgccgg cgcgctcacc gccttctgcg cgctcgccgc
      361 ctgctgaagc gcgagggtgt gctgcaggct gccgacttcg acgagaacgg cctgatcaag
      421 agcctgccca acactccttg gcagctgcgc gctgccgctc tggaccgcaa gctgactcct
      481 ctggagtgga gcgccgtgct gctgcacctg atcaagcacc gcggctacct gagccagcgc
      541 aagaacgagg gcgagaccgc cgacaaggag ctgggtgctc tgctgaaggg cgtggccgac
//
